Li-tubes tsa pokello ea mali tsa EDTA ke life, 'me li sebelisoa joang?

Litekong tsa bongaka le tlhahlobo ea bongaka le kalafo,Li-tubes tsa pokello ea mali tsa EDTA, e le lisebelisoa tsa bohlokoa bakeng sa pokello ea mali, li phetha karolo ea bohlokoa ho netefatsa botšepehi ba lisampole le ho nepahala ha tlhahlobo. Sengoliloeng sena, re tla sekaseka "mohlokomeli ea sa bonahaleng" enoa lefapheng la bongaka ka botlalo ho tsoa likarolong tsa tlhaloso, mofuta oa mebala, molao-motheo oa anticoagulation, sepheo sa tlhahlobo le maemo a ts'ebeliso.

Ke engTube ea pokello ea mali ea EDTA?

Tube ea pokello ea mali ea EDTA ke mofuta oa li-vacuum tsa pokello ea mali e nang le Ethylene Diamine Tetraacetic Acid kapa letsoai la eona, le sebelisoang haholo bakeng sa ho bokella lisampole tsa mali le kalafo ea anticoagulant. EDTA e ka thibela karabelo ea coagulation cascade ka ho chelating li-ion tsa calcium maling, e le ho boloka mali a le maemong a mokelikeli nako e telele, le ho fana ka lisampole tse tsitsitseng bakeng sa liteko tsa tloaelo ea mali le baeloji ea limolek'hule. E fana ka lisampole tse tsitsitseng bakeng sa tloaelo ea mali, biology ea limolek'hule le liteko tse ling.

E le karolo ea bohlokoa ealisebelisoa tsa bongaka, Li-tubes tsa pokello ea mali tsa EDTA li hloka ho ikamahanya le maemo a naha a "lijana tsa pokello ea mali tse sebelisoang e le 'ngoe" (mohlala, GB/T 19489-2008) ho netefatsa ts'ebetso ea sterility, non-pyrogenic le non-cytotoxicity.

Mebala e fapaneng ea li-tubes tsa pokello ea mali tsa EDTA

Ho ea ka litekanyetso tse tloaelehileng tsa machaba (joalo ka tataiso ea CLSI H3-A6), li-tubes tsa pokello ea mali tsa EDTA hangata li koaheloa ka bopherese (EDTA-K2/K3) kapa boputsoa (sodium citrate e kopantsoeng le EDTA) ho khetholla tšebeliso:

Tlhokomeliso: Mefuta e meng e ka 'na ea ngotsoe ka mebala e meng, hlahloba litaelo pele u li sebelisa.

Mokhoa oa anticoagulation oa li-tubes tsa pokello ea mali tsa EDTA

EDTA ka sehlopha sa eona sa limolek'hule tsa carboxyl (-COOH) le li-ion tsa calcium maling (Ca²⁺) li kopantsoe ho theha chelate e tsitsitseng, ka hona e thibela ts'ebetso ea plasminogen, e thibela ts'ebetso ea coagulation ea fibrinogen ho fibrin. Anticoagulation ena e na le litšobotsi tse latelang:

1. ts'ebetso e potlakileng ea ketso: anticoagulation e ka phethoa ka mor'a metsotso e 1-2 ka mor'a ho bokella mali;

2. botsitso bo phahameng: mehlala e ka bolokoa lihora tse fetang 48 (sehatsetsing se ka fetisetsoa lihora tsa 72);

3. Mefuta e mengata ea kopo: e loketse liteko tse ngata tsa hematology, empa eseng bakeng sa liteko tsa coagulation kapa platelet function (li-tubes tsa sodium citrate lia hlokahala).

Lintho tsa mantlha tsa tlhahlobo ea tube ea pokello ea mali ea EDTA

1. tlhahlobo ea mali e tloaelehileng: palo ea lisele tse tšoeu tsa mali, litekanyetso tsa lisele tse khubelu tsa mali, mahloriso a hemoglobin, joalo-joalo;

2. boitsebiso ba sehlopha sa mali le ho kopanya: Sehlopha sa mali sa ABO, ho lemoha Rh factor;

3. tlhahlobo ea limolek'hule: tlhahlobo ea liphatsa tsa lefutso, boikemisetso ba bongata ba kokoana-hloko (mohlala, HIV, HBV);

4. glycated hemoglobin (HbA1c): ho lekola tsoekere ea mali ka nako e telele bakeng sa lefu la tsoekere;

5. tlhahlobo ea likokoana-hloko tsa mali: Plasmodium, ho lemoha microfilariae.

Tšebeliso ea litloaelo le litemoso

1. Mokhoa oa ho bokella:

Ka mor'a ho thibela likokoana-hloko letlalong, sebetsa ho ea ka mokhoa oa ho bokella mali a venous;

Hang ka mor'a ho bokella, fetola tube ea pokello ea mali ka makhetlo a 5-8 ho netefatsa hore anticoagulant e tsoakane ka ho feletseng le mali;

Qoba ho sisinyeha ho matla (ho thibela hemolysis).

2. Polokelo le lipalangoang:

Boloka mocheso oa kamore (15-25 ° C), qoba mocheso kapa serame;

Beha ka holimo nakong ea lipalangoang ho thibela ho lokoloha ha cap cap.

3. maemo a contraindication:

Li-tubes tsa sodium citrate lia hlokahala bakeng sa Coagulation IV (PT, APTT, joalo-joalo);

Teko ea tšebetso ea platelet e hloka sodium citrate tube.

Mokhoa oa ho khetha boleng bo holimoTube ea pokello ea mali ea EDTA?

1. Tšoaneleho le setifikeiti: khetha lihlahisoa tse fetileng ISO13485 le CE setifikeiti. 2;

2. Tšireletseho ea lintho tse bonahalang: 'mele oa tube o lokela ho ba o hlakileng le o se nang masala a polasetiki;

3. Dosing e nepahetseng: palo ea anticoagulant e ekelitsoeng e lokela ho lumellana le maemo a naha (mohlala, mahloriso a EDTA-K2 a 1.8 ± 0.15mg / mL);

4. Botumo ba Brand: Ntho ea pele e fuoa lihlahisoa tse tsebahalang lefapheng la lisebelisoa tsa bongaka ho netefatsa botsitso ba batch.

Qetello

E le setho sa bohlokoa sasesebelisoa sa pokello ea mali, Li-tubes tsa pokello ea mali tsa EDTA li na le tšusumetso e tobileng mabapi le ho nepahala ha sephetho sa liteko ho latela thepa ea bona ea anticoagulant. Ka ho tiisa ts'ebeliso ea li-tubes tsa pokello ea mali tse nang le mebala e fapaneng le ho li kopanya le mekhoa e thata ea ho bokella, e ka fana ka motheo o tšepahalang oa tlhahlobo ea bongaka. Nakong e tlang, ka nts'etsopele ea meriana e nepahetseng, li-tubes tsa pokello ea mali tsa EDTA li tla phetha karolo ea bohlokoa ho feta tlhahlobo ea mali, liphatsa tsa lefutso le likarolo tse ling, 'me li tsoele pele ho sireletsa bophelo ba batho.