Li-tubes tsa ho bokella mali tsa EDTA ke eng 'me li sebelisoa joang?

Litekong tsa bongaka le tlhahlobong ea bongaka le kalafong,Liphaephe tsa ho bokella mali tsa EDTA, e le dintho tsa bohlokwa tse sebediswang bakeng sa pokello ya madi, e bapala karolo ya bohlokwa ho netefatseng botšepehi ba disampole le ho nepahala ha tlhahlobo. Sehloohong sena, re tla sekaseka ka botlalo "mohlokomedi e sa bonahaleng" lefapheng lena la bongaka ho tloha dikarolong tsa tlhaloso, karohano ya mebala, molao-motheo wa thibelo ya ho ruruha ha madi, morero wa teko le maemo a tshebediso.

Ke engPhaephe ea ho bokella mali ea EDTA?

Tube ea pokello ea mali ea EDTA ke mofuta oa tube ea pokello ea mali e nang le Ethylene Diamine Tetraacetic Acid kapa letsoai la eona, e sebelisoang haholo bakeng sa ho bokella lisampole tsa mali le kalafo ea anticoagulant. EDTA e ka thibela karabelo ea coagulation cascade ka ho chelating li-ion tsa calcium maling, e le ho boloka mali a le boemong ba metsi nako e telele, le ho fana ka lisampole tse tsitsitseng bakeng sa liteko tsa mokhoa oa mali le baeloji ea limolek'hule. E fana ka lisampole tse tsitsitseng bakeng sa mokhoa oa mali, baeloji ea limolek'hule le liteko tse ling.

Jwalo ka karolo ya bohlokwa yalintho tse sebelisoang tsa bongaka, Liphaephe tsa pokello ea mali tsa EDTA li hloka ho latela maemo a naha a "Lijana tsa pokello ea sampole ea mali a methapo tse sebelisoang hanngoe" (mohlala, GB/T 19489-2008) ho netefatsa ts'ebetso ea ho hloka nyopa, ho se be le pyrogenic le ho se be le cytotoxicity.

Mebala e fapaneng ea lipeipi tsa ho bokella mali tsa EDTA

Ho ya ka maemo a tlwaelehileng a matjhaba (jwalo ka ditataiso tsa CLSI H3-A6), diphaephe tsa ho bokella madi tsa EDTA hangata di kwahetswe ka pherese (EDTA-K2/K3) kapa boputswa (sodium citrate e kopantsweng le EDTA) ho kgetholla tshebediso:

Ela Hloko: Mefuta e meng e ka 'na ea ngoloa ka mebala e meng, hlahloba litaelo pele u e sebelisa.

Mokhoa oa ho thibela ho ruruha ha mali oa lipeipi tsa pokello ea mali tsa EDTA

EDTA ka sehlopha sa eona sa limolek'hule tsa carboxyl (-COOH) le li-ion tsa calcium maling (Ca²⁺) li kopantsoe ho etsa chelate e tsitsitseng, ka hona li thibela ts'ebetso ea plasminogen, li thibela ts'ebetso ea ho hoama ha fibrinogen ho ba fibrin. Thibelo ena ea ho hoama e na le litšobotsi tse latelang:

1. ho qala ka potlako ha ketso: ho thibela ho ruruha ha mali ho ka phethoa metsotsong e 1-2 kamora ho bokelloa ha mali;

2. botsitso bo phahameng: disampole di ka bolokwa dihora tse fetang 48 (di ka sehatsetsing di ka atoloswa ho fihlela dihora tse 72);

3. Mefuta e mengata ea ts'ebeliso: e loketse liteko tse ngata tsa hematology, empa eseng bakeng sa liteko tsa ho kopana kapa ts'ebetso ea li-platelet (ho hlokahala li-tube tsa sodium citrate).

Lintho tsa bohlokoa tsa tlhahlobo ea tube ea ho bokella mali ea EDTA

1. tlhahlobo ea mali e tloaelehileng: palo ea lisele tse tšoeu tsa mali, litekanyo tsa lisele tse khubelu tsa mali, mahloriso a hemoglobin, jj.;

2. ho tsebahatsa le ho bapisa sehlopha sa mali: Sehlopha sa mali sa ABO, ho lemoha Rh factor;

3. tlhahlobo ea limolek'hule: liteko tsa lefutso, ho fumana mojaro oa vaerase (mohlala, HIV, HBV);

4. hemoglobin e nang le glycated (HbA1c): ho lekola tsoekere maling nako e telele bakeng sa lefu la tsoekere;

5. tlhahlobo ea likokoana-hloko maling: Ho fumanoa ha Plasmodium, microfilariae.

Tšebeliso ea melao le mehato ea ho itšireletsa

1. Ts'ebetso ea pokello:

Kamora ho bolaea likokoana-hloko letlalong, sebetsa ho latela maemo a pokello ea mali a methapong;

Hang ka mora ho bokelloa, fetola tube ya ho bokella madi ka makgetlo a 5-8 ho netefatsa hore anticoagulant e tswakane ka botlalo le madi;

Qoba ho thothomela ka matla (ho thibela hemolysis).

2. Polokelo le lipalangoang:

Boloka mochesong oa kamore (15-25°C), qoba mocheso kapa ho hatsela;

Beha ka ho otloloha nakong ea lipalangoang ho thibela hore sekoahelo sa tube se hlephisoe.

3. maemo a thibelo:

Li-tube tsa sodium citrate lia hlokahala bakeng sa Coagulation IV (PT, APTT, jj.);

Teko ea ts'ebetso ea liplatelete e hloka tube ea sodium citrate.

Mokhoa oa ho khetha boleng bo holimoPhaephe ea ho bokella mali ea EDTA?

1. Litšoaneleho le setifikeiti: khetha lihlahisoa tse fetisitseng setifikeiti sa ISO13485 le CE. 2;

2. Polokeho ea thepa: 'Mele oa tube o lokela ho ba o bonaletsang le o se nang masala a polasetiki;

3. Tekanyo e nepahetseng: bongata ba sethibela-mafu se ekelitsoeng bo lokela ho tsamaellana le maemo a naha (mohlala, mahloriso a EDTA-K2 a 1.8±0.15mg/mL);

4. Botumo ba lets'oao: Bohlokoa bo fuoa mats'oao a tsebahalang lefapheng la lisebelisoa tsa bongaka ho netefatsa botsitso ba sehlopha.

Qetello

Jwalo ka setho sa bohlokwa sasesebelisoa sa ho bokella mali, Lipeipi tsa ho bokella mali tsa EDTA li na le tšusumetso e tobileng ho nepahaleng ha liphetho tsa liteko mabapi le thepa ea tsona ea anticoagulant. Ka ho etsa hore tšebeliso ea lipeipi tsa ho bokella mali tse nang le mebala e fapaneng e be ea 'mala o le mong le ho li kopanya le mekhoa e tiileng ea ho bokella, e ka fana ka motheo o tšepahalang oa tlhahlobo ea bongaka. Nakong e tlang, ka nts'etsopele ea meriana e nepahetseng, lipeipi tsa ho bokella mali tsa EDTA li tla bapala karolo ea bohlokoa haholoanyane tlhahlobong ea mali, tatellano ea liphatsa tsa lefutso le masimo a mang, 'me li tsoele pele ho sireletsa bophelo bo botle ba batho.